Whole Genome Sequencing of Virgibacillus pantothenticus BAB 3240 Isolated from an Indian Saline Desert Reveals Stress Tolerance and Biodegradation Genes

Virgibacillus pantothenticus strain BAB3240, a halotolerant and alkaliphilic bacterium, was isolated from the saline desert soil of Gujarat, India. This study reports the first whole-genome sequencing of V. pantothenticus from India, shedding light on its genetic adaptations to extreme environments. Phylogenetic analysis based on 16S rRNA sequencing confirmed its closest taxonomic affiliation to V. pantothenticus DSM 26, which was further validated by whole-genome sequencing and comparative genomic analysis. The assembled genome (10× coverage) consisted of 229 contigs, with a total length of 4,563,412 bp and an average G + C content of 37.46%. Genome annotation revealed a diverse repertoire of genes associated with osmotic stress adaptation, heavy metal resistance, antibiotic resistance, and xenobiotic degradation pathways, suggesting its potential for bioremediation and industrial applications. Additionally, the strain harbors genes encoding serine alkaline proteases, highlighting its biotechnological relevance for enzyme production under extreme conditions. This genomic resource expands our understanding of Virgibacillus species, their environmental resilience, and their applicability in sustainable bioprocesses.