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September 10, 2025Environmental DNAOpen Access

Towards Comprehensive Metagenomic Recovery and Data Mining of Complex Environmental DNA Viromes

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Evaluation of multiple bioinformatic methods and their effectiveness in viral identification and taxonomy.

Multi-sample assembly significantly improves viral genome recovery compared to single-sample assembly, enhancing accuracy.
VirSorter2 and VIBRANT outperform other tools in identifying viral contigs, delivering superior identification results.
Effective tools like BACPHLIP and PhaTYP successfully differentiate between lytic and lysogenic viruses, aiding classification.
Using varying threshold selections influences viral genome counts while maintaining consistent major patterns in analysis.

Ji et al. (2025) studied this question.

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