The Best Ways to Extract Papaya Seeds for Their Deworming Effects on Ascaridia galli

Previous research has shown that papaya seeds have an anthelmintic effect on Ascaridia galli, but this effect was observed using traditional methods, such as boiling the seeds in water. To determine the most effective extraction method, various techniques were tested, including maceration, soxhlet extraction, and ultrasonic digestion. The study used both wet and dry papaya seeds, with solvents such as water, 70% ethanol, and n-hexane. The anthelmintic activity was measured by the percentage of worm death, LD50, and relative potential compared to pyrantel pamoate, a positive control. The results indicated that the maceration method using 70% ethanol as the solvent for wet papaya seeds was the most effective, killing 60% of the worms with an LD50 of 9.36 mg and a relative potential of 0.66 times that of pyrantel pamoate.