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This protocol facilitates medium throughput protein expression and purification in E. coli, implying efficient preparation for protein screening.
This protocols describes medium throughput protein expression in E. coli using heat-lysis as a built in purification step. It is intended for preparation of heat-stable de novo designed proteins for screening in vitro.1-3 It provides a small but relatively pure lysate sample of the protein within 2 working days.
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Grene et al. (2025) studied this question.
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