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This protocol details the parallel rapid expression and purification of 24x proteins for crystallography (PREPX) at 100 mL culture scale. Recombinant proteins are expressed in Escherichia coli using the autoinduction method and then purified in parallel using a IMAC, desalt, tag cleavage, reverse IMAC and gel filtration work flow.
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M. Fairhead (2024) studied this question.
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