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Rolling Circle Amplification with an Expanded Genetic Alphabet (ExRCA) Generates DNA Materials Incorporating Additional Building Blocks for Enhanced Programmable Functionalization

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ExRCA enhances DNA amplification with unnatural base pairs, suggesting broad applications in biotechnology.

ExRCA facilitates efficient DNA amplification, enhancing function diversity with unnatural base pairs.
The phi29 DNA polymerase shows high fidelity in replicating unnatural base pairs, crucial for ExRCA's success.
ExRCA demonstrates promise in producing multifunctional DNA materials, including hydrogels and catalytic systems.
This method highlights the potential of unnatural base pairs for advanced applications in materials science.

Peng et al. (2025) studied this question.

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