Phytochemical Profiling and Green Synthesis of Zinc Oxide Nanoparticles using Aqueous Extracts of Cynanchumviminale(L.)L. (Apocynaceae)

Cynanchumviminale(L.)L. is a traditional medicinal plant that many people use to treat various illnesses. This study identifies phytochemicals in Cynanchumviminale(L.)L supporting its folkloric use and exploring its potential for safe, accessible ZnOnanoparticle synthesis. Carbohydrates. Reducing sugars, proteins, alkaloids, phenolic compounds, saponins, and flavonoids were all found in the initial phytochemical examination. The quantitative study showed a high concentration of alkaloids (9.2581 mg/g), flavonoids (26.2106 mg/g), and total phenolics (32.731 mg/g). Following nanoparticle manufacturing, phytochemical quantification reveals a reduction in the amounts of flavonoids (6.7324 mg/g) and phenolics (21.320 mg/g). UV-Visible spectroscopy, SEM, and EDX analysis validated the NPs’ production. A zinc oxide nanoparticle-specific absorption peak was detected at 360 nm in the UV-Vis spectrometer. Scanning Electron Microscope (SEM) analysis revealed the presence of zinc oxide nanoparticles. The Energy Dispersive Spectrum (EDX) data indicate a prominent zinc signal at 1.26 keV, signifying the NPS elemental composition and chemical purity. Stabilising agents derived from plant extract were detected in the EDX spectra, particularly carbon, nitrogen, and other elements