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September 10, 2025Indian Journal of Veterinary Research (The)

Protein G chromatography for an efficient purification of monoclonal antibodies

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Authors

SDSonali DubeyCentral University of PunjabSGS. M. GopinathAmrita Institute of Medical Sciences and Research CentreDADas AdwitiyaProject Directorate on Foot and Mouth Disease

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Implication

Observational analysis optimized protein G chromatography for mAbs in hybridoma cultures, indicating enhanced yield.

Key Points

  • The purification method achieved a yield of 1–2.8 mg of immunoglobulins per 100 mL of supernatant.
  • Fine-tuned flow rates, specifically 0.5 mL/min for binding and 1 mL/min for elution, improved recovery rates significantly.
  • The optimized technique utilizes protein G affinity chromatography for effective purification of monoclonal antibodies.
  • This method ensures reliable small-scale purification for downstream applications in various biotechnological processes.

Cite This Study

Dubey et al. (2024) studied this question.

synapsesocial.com/papers/68c1e17054b1d3bfb60fe860https://doi.org/10.5958/0974-0171.2024.00008.x
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