The Role of Diethylphthalate in the Initiation of the Formation of Schiff Bases in the Microsomal Fraction of Rat Livers

The body is exposed to reactive oxygen species (ROS), which are intensively generated in the microsomes of the liver and can initiate the processes of lipid peroxidation (POL) in the membrane structures of the cell. The aim of the work is to evaluate the intensity of formation of the content of the end products of lipoperoxidation in the microsomal fraction of the liver of rats under the action of diethyl phthalate (DEF). The study was conducted on three groups of purebred white rats: Group I – intact animals (K); II group – rats that were orally administered DEF at a dose of 2.5 mg/kg of body weight; Group III - rats that were orally administered DEF at a dose of 5.4 mg/kg of body weight. A microsomal fraction was isolated from the liver of rats, in which the content of Schiff bases was determined as the final products of LPO and TBC-active products as precursor products of Schiff bases. The content of Schiff bases was measured in the heptane phase of lipid extracts of the microsomal fraction of rat liver at a wavelength of 400 nm, TBC-active products at 532 nm. It was established that with the daily introduction of DEF in the diet at a dose of 2.5 mg/kg, an increase in the intensity of the lipoperoxidation process was observed in the microsomal fraction of the liver of rats, as evidenced by an increase in the content of the final products of POL on the 21st day of the experiment. It was shown that with an increase in the dose of DEF administration (5.4 mg/kg), a more significant increase in the content of Schiff bases was observed, since their content on the 14th day of administration of the xenobiotic exceeded 2.5 times, and on the 21st day - by 3.4 times the indicators of intact animals. Therefore, the intensity of lipoperoxidation processes depends on the concentration and timing of DEF entry into the body.