This protocol shows the efficient generation of cDNA from RNA, highlighting its adaptability with common RT enzymes.
This protocol is used for generating cDNA from purified RNA. It is based on RevertAid's manufacturer's protocol, with minor modifications. It can be used with other common RT enzymes such as M-MVL, just make sure to incubate the enzyme at the right temperature. Using this protocol, we have successfully generated cDNA from eukaryotic RNA from several sources. The cDNA can be further used for qPCR, clonning, end-point PCR, etc.
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David Valle‐García (2023) studied this question.
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