Key result
A novel one-tube RT-PCR method successfully amplified a 3-kb genome region from 24 small round-structured virus strains with a detection threshold of 0.2 ng of crude RNA.
A novel one-tube RT-PCR method enables efficient amplification of a 3-kb genome region of SRSVs, facilitating their molecular characterization and diagnostics.
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May enhance SRSV research tools; leaves open clinical validation before diagnostic use.
Ando et al. (1997) studied Small round-structured viruses (Norwalk-like viruses) (n=24). One-tube RT-PCR method was evaluated on Amplification of a 3-kb genome region. A novel one-tube RT-PCR method successfully amplified a 3-kb genome region from 24 small round-structured virus strains with a detection threshold of 0.2 ng of crude RNA.
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