June 18, 2012Microbiology and ImmunologyOpen Access

Amplification of all 11 RNA segments of group A rotaviruses based on reverse transcription polymerase chain reaction

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Population

Seven rotavirus strains (Wa, DS-1, Hochi, 69M, WI61, M37 and SA11-S1) and one fecal specimen

Design

Preclinical

Authors

YFYoshiki FujiiNational Institute of Infectious Diseases TSTakashi ShimoikeNational Institute of Infectious Diseases HTHirotaka TakagiNational Institute of Infectious Diseases

Discussion

Member takes

Implication

May accelerate rotavirus genomic surveillance; leaves open clinical translation pending human validation.

Structured PICO

Population

Seven rotavirus strains (Wa, DS-1, Hochi, 69M, WI61, M37 and SA11-S1) and one fecal specimen

Intervention

A set of universal primers and a high-fidelity and rapid DNA polymerase in a one-step reverse transcription polymerase chain reaction

Outcome

Amplification of almost the entire length of all 11 segments of the rotavirus strainssurrogate

The developed one-step RT-PCR method using universal primers allows for the rapid and accurate amplification of all 11 RNA segments of group A rotaviruses, facilitating complete genome analysis.

Cite This Study

Fujii et al. (2012) studied this question.

synapsesocial.com/papers/6a6fb25aa2d7cf2e39c294a3 https://doi.org/10.1111/j.1348-0421.2012.00479.x

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Amplification of all 11 RNA segments of group A rotaviruses based on reverse transcription polymerase chain reaction

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Amplification of all 11 RNA segments of group A rotaviruses based on reverse transcription polymerase chain reaction

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider