Key result
Monoplex nested RT-PCR yields a ~24% higher rotavirus G genotype detection rate than multiplex testing.
Why the study?
Rotavirus group A is a major cause of acute gastroenteritis in children under 5 years and requires molecular characterization of G and P genotypes for epidemiological understanding.
Cross-Sectional (n=274)
Absolute Event Rate: 57.6% vs 46.4%
p-value: p=<0.0001
Ongoing rotavirus surveillance in children under 5 remains essential; leaves open reassortment impacts on vaccine performance in diverse settings.
INTRODUCTIONRotaviruses belong to the genus Rotavirus within the Reoviridae family and its genome consists of eleven dsRNA segments.These viruses are distinct in that their segmented genome undergoes reassortment during replication.1 According to the classification system based on the gene sequence of VP6 an inner capsid protein, rotaviruses are currently categorized into nine groups (A-I). 2 Rotavirus group A is a major etiologic agent of acute gastroenteritis in children under 5 years of age and is associated annually with approximately 215,000 deaths worldwide. 3Rotaviruses group A are classified based on the molecular characterization of the two outer capsid proteins, VP7 (glycoprotein) and VP4 (protease-sensitive) into different G and P genotypes respectively.4 At least 36 G genotypes and 51 P genotypes of group A rotavirus had been reported to cause .
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El‐Senousy et al. (2020) conducted a cross-sectional in Human rotavirus group A infection (n=274). Monoplex nested and semi-nested RT-PCR vs. Multiplex nested and semi-nested RT-PCR was evaluated on Detection of rotavirus common G genotypes in clinical specimens (p=<0.0001). Monoplex nested RT-PCR detected rotavirus common G genotypes in 57.60% of clinical specimens, significantly higher than the 46.40% detected by multiplex nested RT-PCR (p<0.0001).
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