Two Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strains, designated SZ-1-7 T and 4WD22 T , were isolated from a mangrove swamp in Shenzhen and a marine solar saltern in Weihai, respectively. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SZ-1-7 T and strain 4WD22 T belong to the phylum Bacteroidota . The percentage of conserved protein and average nucleotide identity values between strain SZ-1-7 T and the genus Tamlana were 56.6–74.0% and 74.7–90.5%, respectively. Strain SZ-1-7 T grew from 15 to 40 °C (optimally at 33 °C), pH 6.0–10.0 (optimally at 7.0–8.0) and in the presence of 0–7% (w/v) NaCl (optimally in 2% NaCl). Strain SZ-1-7 T could produce carotenoid-type pigments. Strain 4WD22 T grew from 20 to 45 °C (optimally at 40 °C), pH 6.0–10.0 (optimally at 8.0) and in the presence of 1–5% (w/v) NaCl (optimally in 2% NaCl). MK-6 was the only respiratory quinone that was compatible with the genus of strain SZ-1-7 T . The strain 4WD22 T contained MK-7 as the predominant menaquinone. The major polar lipids of strain SZ-1-7 T were three kinds of unidentified lipids, five kinds of unidentified aminolipid and phosphatidylethanolamine, and those of strain 4WD22 T were phosphatidylethanolamine, three unidentified aminolipids and five unidentified lipids. The strain SZ-1-7 T was verified to have the potential to degrade polysaccharides in this study. The predatory capability of strain 4WD22 T was tested in this study. The DNA G+C contents of strain SZ-1-7 T and strain 4WD22 T were 36.5 and 41.5 mol%, respectively. Based upon the results presented in this study, strain SZ-1-7 T and strain 4WD22 T represent novel species of the genera Tamlana and Catalinimonas , respectively, for which the names Tamlana sedimenti sp. nov. and Catalinimonas locisalis sp. nov. are proposed with the type strain SZ-1-7 T (=MCCC 1H01428 T =KCTC 102108 T ) and strain 4WD22 T (=MCCC 1H01431 T =KCTC 102105 T ).
Liu et al. (Thu,) studied this question.