Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
September 12, 2025

In-Depth Analysis of Data Characteristics and Comparative Evaluation of DDA and DIA Accuracy in Label-Free Quantitative Proteomics of Biological Samples

View Full Paper
Ask AI
Bookmark
Share

Authors

XZXun ZouLWLulu WangYCYulu Chen

Discussion

Loading...

Member takes

Overview

Systematic comparison reveals DIA's superiority in protein identification and quantitative coverage for biological samples.

Key Points

  • DIA outperformed DDA in protein identification, with 7,740 versus 5,159 detected proteins in disease samples, showing a significant improvement.
  • Average quantifiable protein ratios were higher for DIA at 98–99% compared to 92–95% for DDA, demonstrating superior quantitative coverage.
  • The analysis included data completeness and reproducibility, with DIA exhibiting intragroup correlation coefficients greater than 0.98, enhancing data reliability.
  • Discrepancies between DIA and DDA were mainly due to the exclusion of single-peptide proteins, improving overall data quality and insight accuracy.

Cite This Study

Zou et al. (2025) studied this question.

synapsesocial.com/papers/68d44b2a31b076d99fa543f9https://doi.org/10.21203/rs.3.rs-7430109/v1
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Interrogating Data-Independent Acquisition LC-MS/MS for affinity proteomics2024
  2. 2Data-Independent Acquisition Mass Spectrometry as a Tool for Metaproteomics: Interlaboratory Comparison Using a Model Microbiome2024 · 6 citations
  3. 3A New Evaluation Metric for Quantitative Accuracy of LC–MS/MS-Based Proteomics with Data-Independent Acquisition2024 · 5 citations
  4. 4Reanalysis of DIA Data Demonstrates the Capabilities of MS/MS-Free Proteomics to Reveal New Biological Insights in Disease-Related Samples2024 · 6 citations
  5. 5iDIA-QC: AI-empowered data-independent acquisition mass spectrometry-based quality control2025 · 10 citations