What type of study is this?

This is a In Vitro Study study.

September 12, 2025Weed ScienceOpen Access

Pro-197-Thr mutation in ALS gene confers higher pyroxsulam resistance than Pro-197-Gln in Italian ryegrass (Lolium perenne ssp. multiflorum)

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The study identifies distinct ALS gene mutations affecting herbicide resistance levels, suggesting unique resistance mechanisms in Italian ryegrass.

The Pro-197-Thr mutation results in a significantly higher resistance index to pyroxsulam compared to Pro-197-Gln.
Whole-plant bioassays revealed the Pro-197-Thr population had an RI of 508.92 versus 9.75 for Pro-197-Gln.
In vitro ALS assays confirmed that the Pro-197-Thr population is less sensitive to pyroxsulam compared to the Pro-197-Gln population.
Findings indicate that different amino acid substitutions at a single ALS gene locus can significantly affect herbicide resistance mechanisms.

Yin et al. (2025) studied this question.

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The study identifies distinct ALS gene mutations affecting herbicide resistance levels, suggesting unique resistance mechanisms in Italian ryegrass.