Chromosome-level genome assembly of the big-footed bat (Myotis pilosus)

Abstract Some bat species in the genus Myotis have evolved longevity-associated mechanisms and exhibit remarkable resistance to cancer. Among them, the big-footed bat (Myotis pilosus) has been confirmed as a cancer-resistant species. Here, we assembled a chromosome-level genome of the big-footed bat, utilizing a combination of ONT long reads and Hi-C technologies. The size of this genome is 1968.27 Mb, with a contig N50 of 41.29 Mb. All assembled sequences were anchored onto 21 autosomes and X chromosome. We identified 739.02 Mb (37.55%) of repetitive sequences in the genome and predicted 21,368 protein-coding genes. Assessment of the genome assembly quality indicated that the assembled genome of the big-footed bat exhibits excellent continuity, completeness, and accuracy. Taken together, we have generated the first chromosome-level genome assembly of the big-footed bat, providing an important reference resource for genetic and genomic studies of long-lived bat species.