Cookbook for Plant Genome Sequences

Access to genome sequences is crucial to investigate and engineer traits in plants, explore biodiversity, and support phylogenetic studies. During the last decade, affordable sequencing devices have substantially increased the size of the genomics community by enabling individual research groups to sequence the genomes of their favourite plants. There has also been a quick development of novel tools for sequencing data analysis. Here, we share experiences with plant long-read genomics methods for Oxford Nanopore Technologies (ONT) and provide hands-on guidelines to support newcomers’ dive into plant genomics. The full workflow from planning a plant genome sequencing project to public release of associated data sets is covered.