Study of Culture Sensitivity of Semen in Infertility Cases – Retrospective Study

Background: Male infertility is noted Up to 50% vitro studies revealed that, bacteria can negatively impact on sperm function. Hence the human microbe and dysbiosis have to be ruled out. Method: 65 (sixty-five) infertile adults aged between 25 to 40 years were studied. Semen samples were collected in a sterile container by masturbation after the minimum obstinate of 3 days. Semen parameters included appearance, volume, pH, viscosity, liquefaction, motility, and morphology, which were analyzed microscopically. Results: Out of 65, 55 (84.6%) were primary infertility, and 10 (15.3%) were secondary infertility. In a comparative study of semen parameters of bacteriological culture of positive and negative studies, mean volume, pH, sperm concentration, progressive motility, normal formation of sperm, had significant p-value (p<0.001). In comparison of PCR semen concentration, progressive motility morphology had a significant p-value (p<0.01). Conclusion: Present bacteriological and PCR studies will help the clinician to rule out the etiology of abnormal semen parameters and to treat efficiently such infertile male cases