What question did this study set out to answer?

To characterize and compare the bacterial communities of sperm samples from infertile men and determine their relationship with sperm count, motility, and morphology.

March 9, 2022Journal of Clinical MedicineOpen Access

Spermatic Microbiome Characteristics in Infertile Patients: Impact on Sperm Count, Mobility, and Morphology

View Full Paper

Ask AI

Bookmark

Authors

CGClémence Gachet MPManon Prat CBChristophe Burucoa

Discussion

Member takes

Overview

Prospective cohort study reveals altered seminal bacterial diversity and composition in infertile men, indicating that modulating genital microbiota may improve reproductive outcomes.

Key Points

To characterize and compare the bacterial communities of sperm samples from infertile men and determine their relationship with sperm count, motility, and morphology.
Conducted a prospective, monocentric study from September 2019 to March 2020 evaluating semen samples from patients undergoing couple infertility diagnosis (N=91, including 53 with sperm abnormalities).
Amplified the 16S rDNA hypervariable regions (V1 to V3) and sequenced bacterial libraries using the Illumina MiSeq platform.
Analyzed sequence data via a quality control pipeline utilizing QIIME2 modules to assess taxonomic composition, alpha-diversity, and beta-diversity across spermatic parameters.
The dominant bacterial genera across semen samples were Prevotella, Finegoldia, Pseudomonas, Peptinophilus, Streptococcus, Anaerococcus, and Corynebacterium.
Samples from patients with abnormal sperm morphology showed significantly restricted alpha-diversity (p < 0.05), whereas samples with abnormal sperm counts exhibited significantly altered beta-diversity (p < 0.05).
Significant taxonomic shifts occurred across subgroups, including enrichment of Prevotella in negative sperm culture samples and Haemophilus in samples with abnormal sperm counts (p < 0.05).

Cite This Study

Gachet et al. (2022) studied this question.

synapsesocial.com/papers/69de7a157ed287395e558fa8 https://doi.org/10.3390/jcm11061505

View Full Paper

Ask AI

Bookmark

March 9, 2022Journal of Clinical MedicineOpen Access

Spermatic Microbiome Characteristics in Infertile Patients: Impact on Sperm Count, Mobility, and Morphology

View Full Paper

Ask AI

Bookmark

Authors

CGClémence Gachet MPManon Prat CBChristophe Burucoa

Discussion

Member takes

Overview

Prospective cohort study reveals altered seminal bacterial diversity and composition in infertile men, indicating that modulating genital microbiota may improve reproductive outcomes.

Key Points

To characterize and compare the bacterial communities of sperm samples from infertile men and determine their relationship with sperm count, motility, and morphology.
Conducted a prospective, monocentric study from September 2019 to March 2020 evaluating semen samples from patients undergoing couple infertility diagnosis (N=91, including 53 with sperm abnormalities).
Amplified the 16S rDNA hypervariable regions (V1 to V3) and sequenced bacterial libraries using the Illumina MiSeq platform.
Analyzed sequence data via a quality control pipeline utilizing QIIME2 modules to assess taxonomic composition, alpha-diversity, and beta-diversity across spermatic parameters.
The dominant bacterial genera across semen samples were Prevotella, Finegoldia, Pseudomonas, Peptinophilus, Streptococcus, Anaerococcus, and Corynebacterium.
Samples from patients with abnormal sperm morphology showed significantly restricted alpha-diversity (p < 0.05), whereas samples with abnormal sperm counts exhibited significantly altered beta-diversity (p < 0.05).
Significant taxonomic shifts occurred across subgroups, including enrichment of Prevotella in negative sperm culture samples and Haemophilus in samples with abnormal sperm counts (p < 0.05).

Cite This Study

Gachet et al. (2022) studied this question.

synapsesocial.com/papers/69de7a157ed287395e558fa8 https://doi.org/10.3390/jcm11061505

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark