Sources and Transport of Dissolved Organic Matter (DOM) in Surface and Groundwater Within a Dominated Greenhouse Agriculture Catchment: Insights from Multi-Tracer

Intensive greenhouse agriculture significantly alters dissolved organic matter (DOM) dynamics in aquatic ecosystems, but related research remains scarce. To address this knowledge gap, this study employed an integrated approach combining Excitation–Emission Matrix Parallel Factor Analysis (EEM-PARAFAC), Two-Dimensional Correlation Spectroscopy (2D-COS), and Self-Organizing Map (SOM) analyses with hydrochemical and stable water isotopes (δ18O and δD) to investigate the dynamic characteristics of DOM in surface water and groundwater in an intensive greenhouse agriculture catchment (XER) in northern China. Water chemistry and isotope results consistently demonstrated mixing between surface water and groundwater, which was attributed to irrigation pumping. Four fluorescent components were identified via EEM-PARAFAC (C1 and C4 are humic components, while C2 and C3 are tryptophan components), with microbial decomposition of organic fertilizers and domestic wastewater discharge being important sources. Compared with tryptophan components, terrestrial humic substances in groundwater preferentially change in the parallel river direction, while microbial humic substances are more sensitive in the vertical direction, as confirmed by 2D-COS. SOM analysis validated the EEM-PARAFAC results through component plane visualization, demonstrating both DOM inter-component relationships and their correlations with inorganic ions. These results provide critical scientific support for developing sustainable water resource management strategies and optimizing organic fertilizer use in greenhouse agricultural systems, with important practical implications for protecting groundwater quality in intensively cultivated catchments.