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This is a Experimental Study study.

September 16, 2025Microbiology SpectrumOpen Access

Identification of dehydrogenase, hydratase, and aldolase responsible for the propionyl residue removal in degradation of cholic acid C-17 side chain in Comamonas testosteroni TA441

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Observational analysis identified dehydrogenase and hydratase in Comamonas testosteroni, suggesting similarities to Mycobacterium tuberculosis.

Demonstrated the roles of ORF genes, revealing ORF41/ORF44 and ORF40/ORF42 as dehydrogenase and hydratase.
Identified three enzymes responsible for propionyl residue removal in the degradation of the C17 side chain.
Analysis using gene-disrupted mutants verified the functional roles of key enzyme complexes, highlighting a unique structural conservation.
Insights into steroid-degrading enzymes may help understand Mycobacterium tuberculosis metabolism and implications for health.

Masae Horinouchi (2025) studied this question.

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Observational analysis identified dehydrogenase and hydratase in Comamonas testosteroni, suggesting similarities to Mycobacterium tuberculosis.