Evaluation of biomaterial containing alginate and egg-shell membrane incorporated with β-tricalcium phosphate for treatment of bone defects

The freeze-drying procedure was used to create the scaffold that was meant to cure bone deformities. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction analysis, swelling and degradation ratio, and scanning electron microscopy (SEM) tests were used to characterize the scaffolds. Osteoblast cells were used in an in vitro cytotoxicity test to evaluate the scaffolds' biocompatibility. The results of the characterization revealed an interconnected porous structure and significant ionic connections between the alginate, egg-shell membrane, and β-tricalcium phosphate (β-TCP). The scaffolds are regarded as nontoxic because the cell viability was more than 80%. When all the findings are evaluated, β-TCP with alginate/egg-shell membrane can be regarded as appropriate for bone treatment applications.