UV-induced accumulation of callosоe in winter wheat plants

Aim. Winter wheat (Triticum aestivum L.) is an important agricultural crop. Its resistance to stress factors and environmental changes significantly affect the yield, and the study of resistance mechanisms is relevant. The aim of the work is to determine the amount of constitutive and UV-B induced accumulation of callose in winter wheat seedlings of Myronivska 808 variety and to find out the effect of UV-B irradiation doses on their growth parameters. Methods. Plants were exposed to UV-B at doses of 0.1, 1 and 10 kJ/m2, growth parameters were measured and the amount of accumulated callose was determined by the Kauss method. Results. The growth parameters and the amount of accumulated callose under UV-B irradiation were determined. A comparative analysis of growth parameters and the results of constitutive and induced calose accumulation was performed. Conclusions. After irradiation, the growth parameters increased in proportion to the received dose of UV-B. In the dose range from 0.1 to 10 kJ/m2, there is a stimulating effect of their growth. The average value of calose accumulation was about 0.8 μg-eq/mg cmrm, and its amount had the character of an oscillatory increase/decrease circuit with a gradual increase in its amount in proportion to the dose. The maximum amount of callose (1,8–2,2 μg-eq/mg cmrm) was found in samples irradiated with a dose of 10 kJ/m2 . The decrease in the amount of calose at 72 and 120 h is probably due to biochemical transformations in the structures of cell walls.