Clinical and Hematological Features of Acute Leukemia in Pediatric and Adult Patients at a Tertiary Care Hospital Lahore

Background and Objectives: Terpinen-4-ol (T4-ol) is a monoterpene derivative of essential oil which hasmany anti- inflammatory, bactericidal and antifungal activities and is safe as compared to other treatmentoptions available. Our study's objectives were to analyze the effects of different concentrations of Terpinen4-ol on apoptosis of cell lines.Methods: This in vitro experimental study was conducted at the IMBB Department, University of Lahore,Lahore Pakistan, from October 2022 to March 2023. Study treatment was divided into four groups: negativecontrol (normal cell line), positive control (cisplatin) Terpinen-4-ol (experimental group) and vehicle control(DMSO). In order to culture cells cryovials were thawed and then cells were cultured in T75 flasks. To assessthe cell viability MTT assay was used. Bioassay technology laboratory ELISA kit was used to evaluateinflammation and apoptosis. Graph Pad prism was used to perform statistical analysis. The data wasanalyzed using one- way analysis of variance (ANOVA) with bonferroni comparison within groups. P ≤ 0.05was considered statistically significant.Results: When compared to the control group, the treated lines showed more apoptotic changes. P-value was≤ 0.05.Conclusion: In this study we have observed anticancer effects of T4-ol against GBM cell line by observingp53 induced apoptosis.