In-Vitro Growth Pattern Estimation of Chickpea (Cicer arietinum L.) using Plant Growth Promoting Rhizobacteria (PGPR)

Nutrient deficiency in the agricultural field gives rise to a challenge for the production of grains at the global level. The utilization of chemical fertilizers to boost crop yield in the present causes detrimental effects on the health of biological communities, including the human population. Therefore, the present research has been designed to isolate two strains, i.e., Pseudomonas and Azotobacter, from the rhizospheric soil of Cajanus cajan (pigeon pea). Both strains of the PGPRs were characterized by performing morphological and biochemical analysis. The inoculation of isolated PGPR bacteria and their effect on Cicer arietinum (chickpea) were conducted under a plant growth chamber where C. arietinum plants were grown in three cups containing autoclaved soil, and each cup was marked for Pseudomonas, Azotobacter, and one for control. Separately, Pseudomonas, Azotobacter cultures were inoculated in two flasks containing 250 ml autoclaved distilled water, and one was used as a control. Incubate the inoculated flasks at 37℃ for three days. Each 50ml suspension was added to each marked cup after a three-day interval. Observed the efficacy of inoculation with PGPR strains separately. In the present study, the root and shoot length of the C. arietinum plant in the presence of bacterial strains were studied. The maximum growth occurs in the Pseudomonas-treated cup in comparison to the Azotobacter. The growth of the C. arietinum plant occurs due to the plant growth-promoting activity of these bacteria. Therefore, bacterial inoculation should be an effective biofertilizer for the growth of C. arietinum.