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September 15, 2025Open Access

Illumina Dual Index Amplicon Sequencing Sample Preparation COI Leray-XT primer set v1

E. Stensrud Alexander Eiler

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Key Points

Efficient preparation of PCR products enhances the accuracy of amplicon sequencing using COI-Leray XT primers.
The use of dual index strategy in Illumina sequencing allows for better sample identification and multiplexing.
Optimized sample preparation methods are crucial for successful amplicon sequencing and downstream applications.
The COI-Leray XT primer set is specifically designed for versatile applications in environmental DNA studies.

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Implication

Protocol details PCR product preparation for amplicon sequencing using COI-Leray XT primers, highlighting efficiency.

Abstract

Preparation of PCR products for amplicon sequencing using COI-Leray XT primers.

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Stensrud et al. (2025) studied this question.

synapsesocial.com/papers/68d454c531b076d99fa5a13c https://doi.org/10.17504/protocols.io.e6nvw4z2dlmk/v1

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