This protocol is used for eDNA metabarcoding of the Cytochrome Oxidase I (COI) "Leray fragment", using Pair-End Illumina MiseqSequencing. As part of the Hakai Institute Ocean Observing Program, biomolecular samples have been collected weekly, from 0 m to near bottom (260 m), to genetically characterize plankton communities in the Northern Salish Sea since 2015. This protocol is developed to give a species-level resolution of marine invertebrates.
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Carvalho et al. (2024) studied this question.
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