Cytotoxicity study of transdermal desloratadine delivery system on murine melanoma and human dermal fibroblast cell cultures

treat and prevent allergic conditions, reducing the adverse effects associated with oral administration. Materials and Methods: We evaluated the cytotoxicity of the transdermal desloratadinedelivery system in micellar and ionic forms on murine melanoma and human dermal fibroblast cell cultures using the MTT assay and resazurin live cell assay. Results: The results of the MTT assay demonstrated that desloratadine in micellar and ionic forms had a more pronounced cytotoxic effect on murine melanoma cells than on human dermal fibroblasts. The transdermal system had no effect on cell viability. Desloratadine in micellar or ionic form reduced cell viability: the survival rate of murine melanoma cells was below 50%, while when incubated with the transdermal system, the viability of human dermal fibroblasts was above 70%, indicating no toxicity. Discussion: The polyvinylpyrrolidone-based transdermal system is not cytotoxic, but the active ingredient, desloratadine, features antiproliferative activity, to a greater extent, in relation to tumor cells. Conclusion: The obtained results demonstrated the cytotoxic effect of micellar and ionic desloratadine on the tumor culture of murine melanoma and the biocompatibility of the transdermal desloratadine delivery system with human dermal fibroblasts.