Modified buffer improves fungal DNA extraction efficiency, suggesting greater stability at room temperature.
This is a modified version of an extraction buffer that is widely used for fungal DNA extraction. It works very well. The high pH and EDTA will protect DNA from nuclease activity, even at room temperature for long periods of time. It is inexpensive and uses common reagents to make. A short example method is provided. Here is another protocol that is used by the Fungal Diversity Survey. dx.doi.org/10.17504/protocols.io.e6nvw1y59lmk/v2
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Russell et al. (2025) studied this question.
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