Spatial biomechanics quantification of the Tumoural MicroEnvironment of a HepG2 spheroids with Magnetic Resonance Elastography

Motivation: The Tumoural-Micro-Environment (TME) is characterized by an increase in stiffness, hence the motivation to consider MR-Elastography for its characterization. Goal(s): We present an experimental setup to locally characterize the biomechanics of cancer cells spheroid embedded in a collagen matrix using Magnetic Resonance Elastography (MRE). Approach: Using MRE at 35 microns resolution, we can locally quantify the biomechanic of a spheroid embedded in a collagen matrix. Results: Comparing the biomechanical properties of a tumour spheroid with an agar spheroid, we show that tumour spheroid modulates their microenvironment within a perimeter of 0.9mmx1.8mm. Impact: Developing 3D culture model, starting from patient's biopsy samples, that is predictive to their in vivo response to treatment, easily applicable could be relevant in the field of tailored medicine. This could spare the patient non-effective chemotherapy exposure or surgery.