In vivo Detection of Mouse Visual Sensory Pathways using Multi-Slice DIANA fMRI

Motivation: Despite extensive research, an established noninvasive mapping of the mouse visual pathway, particularly in higher-order areas, remains lacking. Goal(s): This study aimed to elucidate the mouse visual pathway in vivo, including higher-order areas. Approach: Here, we performed multi-slice DIANA fMRI at 9.4T to identify visual areas through spatiotemporal activation mapping. Results: Significant DIANA responses (0.15-0.25%) were observed in the LGd, VISp, VISa, and VISpm in response to flashing light. The sequence of peak latencies was aligned with the established hierarchical organization of the mouse visual areas. This study highlights DIANA's potential to identify visual-related brain circuits and contribute to dynamic brain network modeling. Impact: The implementation of multi-slice DIANA fMRI with millisecond temporal resolution is expected to enhance our understanding of mouse visual pathways, revealing new insights into brain circuitry and supporting the development of dynamic brain network models.