Optimization of Antioxidant Extraction From Moringa oleifera Seeds Using Response Surface Methodology: Phytochemical Analysis and DPPH Assay

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This analysis uncovers optimal extraction parameters for antioxidant compounds in moringa oleifera seeds, suggesting significant health benefits.

Optimal extraction conditions for antioxidants from Moringa oleifera seeds were identified as 42.5°C, 40% ethanol, and 35.5 hours.
The extracts exhibited significant antioxidant activity with an IC50 of 15.80 mg/mL compared to ascorbic acid at 12.60 mg/mL.
Phytochemical analysis revealed low free fatty acid content and a high degree of unsaturation in Moringa oleifera seed extracts.
Gas chromatography–mass spectrometry identified several bioactive compounds that contribute to the strong antioxidant capacity of the extracts.

Geleta et al. (2025) studied this question.

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This analysis uncovers optimal extraction parameters for antioxidant compounds in moringa oleifera seeds, suggesting significant health benefits.