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September 17, 2025ACS SensorsOpen Access

Structural and Kinetic Profiling of Rolling Circle Amplification via Solid-State Nanopore Sensing Using miR-21 as a Model

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Authors

KLKawin LohaTBThitikorn BoonkoomHPHarit Pitakjakpipop

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Overview

This study demonstrates the use of rolling circle amplification and nanopore sensing to analyze nucleic acids, suggesting new diagnostic applications.

Key Points

  • The study shows time-dependent increases in translocation dwell time and current blockage during amplification.
  • Observations revealed consistent generation of longer and more complex DNA concatemers across RCA incubation periods.
  • Urea-PAGE and atomic force microscopy validated the findings, indicating stable structural configurations over time.
  • Integration of signal extraction applications enhances reproducibility in event classification and visualization.

Cite This Study

Loha et al. (2025) studied this question.

synapsesocial.com/papers/68d45e6a31b076d99fa5efa5https://doi.org/10.1021/acssensors.5c02039
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