Effect of Different Luteinizing Hormone Releasing Hormone Analogs (LHRHa) on Spawning in Mahseer barb (Neolissochilus stracheyi)

The study assessed the impact of varying doses of luteinizing hormone releasing hormone analogs (LHRHa) on key reproductive parameters in Mahseer barb fish (N. stracheyi). Four treatment groups were established. Trt1 (control) received 0.9% sodium chloride of 0.3 mL/kg BW with 10 mg domperidone (Dom)/kg BW; Trt2 was administered 20 μg/kg BW of LHRHa with 10 mg Dom/kg BW; Trt3 received 25 μg/kg BW of LHRHa with 10 mg Dom/kg BW; and Trt4 was given 30 μg/kg BW of LHRHa with 10 mg Dom/kg BW. Each treatment was replicated three times with three fish per replicate. The outcomes revealed a 100% success rate in ovulation induction across all groups using LHRHa with Dom. Trt4, with the highest dosage, achieved the most notable results, showing an ovulation index of 95.12 ± 2.54%. In contrast, the control group (Trt1) recorded the lowest ovulation index at 6.10 ± 0.73%. The application of 30 μg/kg BW of LHRHa with 10 mg Dom/kg BW in Trt4 resulted in the quickest latency period of 30.35 ± 0.60 hours and significantly improved the fertilization rate compared to the lower dosages used in Trt2 and Trt3. However, Trt4 not only demonstrated the highest efficacy in terms of latency, number of ovulated eggs, fertilization rate, hatching rate, and survival rate but also confirmed the superior potential of the highest LHRHa dosage of 30 μg/kg BW combined with Dom for successful spawning induction in Mahseer barb fish.