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September 17, 2025Open Access

Development of a Toolkit for High-Efficiency, Markerless, and Iterative Genome Editing in Shouchella clausii

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Authors

CCClaudia CappellaCJCarsten JersLNLorenzo Ninivaggi

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Overview

The toolkit enables genetic transformation and editing in Shouchella clausii, suggesting broad utility in synthetic biology applications.

Key Points

  • Markerless genome editing achieved over 60% success in targeting non-essential genes in S. clausii.
  • A versatile toolkit demonstrated compatibility with Golden Gate assembly for easy plasmid construction.
  • The system allows for scar-free point mutations and gene replacements, enhancing bacterial engineering potential.
  • Applications extend to Bacillus subtilis, showcasing widespread utility in Gram-positive bacteria.

Cite This Study

Cappella et al. (2025) studied this question.

synapsesocial.com/papers/68d45e6a31b076d99fa5f088https://doi.org/10.1101/2025.09.09.675032
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