Effect of Two Fertilization Media with Different Amino Acid Compositions on the Fertilization Rate and Embryo Quality After Conventional IVF

Introduction: The choice of embryo culture medium is a critical factor influencing multiple aspects of in vitro fertilization (IVF) cycles, including fertilization rate, embryo development, and overall treatment cycles. However, the detailed composition of many commercially available media remains undisclosed, and thus their effects have not been thoroughly analyzed. Aims: This study aimed to investigate whether two types of fertilization media with different amino acid compositions affect the fertilization and embryo quality and sperm motility characteristics after conventional IVF (C-IVF). Methods: This retrospective cohort study was conducted at a single IVF center between January 2023 and November 2023. We included 389 cycles fertilized by C-IVF. Oocyte retrieval cycles were assigned into the G-IVF Plus and IVF Plus groups. We compared the normal fertilization rate after C-IVF and blastocyst development, the good morphology blastocyst rate ([Formula: see text]BB), clinical outcomes, and sperm motility characteristics between the groups. Results: The normal fertilization rate was significantly higher in the IVF Plus group than in the G-IVF Plus group (69.6% vs. 74.2%, [Formula: see text] 0.0110). In addition, sperm superior curvilinear velocity (VCL) and amplitude lateral head displacement (ALH) were maintained after 3-4 hours of incubation. However, there was no significant difference in the blastocyst rate or the good morphology blastocyst rate or clinical outcomes between the IVF media. Conclusions: This study suggests that the use of IVF Plus improves the normal fertilization rate after C-IVF. The differences in amino acid composition may affect the normal fertilization rate and sperm motility characteristics after C-IVF.