This protocol demonstrates agarose-gelatin embedding to improve tissue handling and preservation in samples, highlighting its benefits over traditional methods.
This protocol details an agarose–gelatin double embedding workflow to immobilize small tissue fragments or spheroids for routine paraffin embedding. Samples are embedded in a 2% agarose–5% gelatin mixture, then fixed in neutral buffered formalin (NBF) and processed by standard FFPE methods. Compared with conventional methods, it facilitates handling of small specimens, preserves orientation, and minimizes sample damage.
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Maeng et al. (2025) studied this question.
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