Immunity genes and intestinal microbiocenosis of rabbits when using the probiotic drug «Vetom 2»

The relevance of this study is due to the fact that rabbits often have digestive problems when their type of diet changes. Studying the presence of certain alleles of immune genes and the relationship with the composition of the intestinal microbiome will help identify more stable genotypes of animals. The aim of the research was to study the presence of certain immune alleles and the composition of the intestinal microbiome of rabbits when using a probiotic supplement containing bacteria of the Bacillus amyloliquefaciens strain ("Vetom 2") by molecular methods. Genomic DNA was isolated from the intestines of rabbits using the ExtractDNABlood&Cells. Amplification of variable regions V3-V4 gene of the 16S rRNA using universal primers and subsequent sequencing on the IlluminaMiSeq platform and bioinformatics analysis (clustering, OUT taxonomic classification, and visualization of diversity) were performed in the QIIME2 condition. The dominant species is Campylobacter_D cuniculorum (family Campylobacteraceae , type Pseudomonadota ), and its proportion is higher in the intestinal microbial community of rabbits of the control group by 1.5-4.5 p.p. than that of animals of the experimental group. Acinetobacter is also more widely represented in the control group. The intestines of rabbits of both control and experimental groups contain approximately the same amount of Psychrobacter faecalis and Akkermansia. Faecousia, Lachnospiraceae, Oscillospiraceae, Cryptobacteroides, Oscillospiraceae, Escherichia, Oscillospiraceae, and Gemmiger are more widely represented in the experimental group. PCoA analysis showed that the groups «Chyme» (Rab) and "Mucosa" (Rab1), with rare exceptions, fall into the same clusters.