Background: It has been proven that adding date kernel extract to cosmetics as therapeutic supplements, due to antioxidant and antiviral content, is safe and effective in rejuvenating patients' faces .Objectives: This study aims to evaluate the effectiveness and safety of a formulated cosmetic gel (CG), and date palm kernel methanol extract (DPKME) alone and in combination with FCG@DPKME in treating and enhancing facial skin quality in women using a protocol developed by local cosmetic experts.Patients/Methods: In this study, Herpes virus was isolated and identified using biological and molecular methods from clinical specimens collected from pus and fluids from gynecological patients with edema, erythematous boils, and vesicles. Using Gas chromatography-mass spectrometry (GC–MS) analysis of DPKME. A combination formula Face cosmetic gel (CG), and date palm kernel methanol extract (FCG@ DPKME) prepared and applied externally directly to the skin surface of female patients weekly and continued for 14 weeks. Evaluations included therapeutic assessments of edema, erythematous boils, and biophysical assessments covering the four emerging perceptual categories (EPCs) of skin quality and a subjective assessment using the Global Aesthetic Improvement Scale.Results: The isolated virus was identified as Herpes Simplex Virus-1(HSV -1 )using PCR by specific primers with 99.25 % similarity, related to HSV strains recorded PLAST in Gene Bank . HSV -1 treated with Face cosmetic gel (CG), date palm (phoenix dactylifera L.) kernel methanol extract CG@DPKME in Vero cell line in vitro. CG@DPKME showed longevity and reduced virus titer by 95.5% compared to 90.5% with date Palm kernel methanol extract (DPKME) alone and 45.7% with the basic cosmetic gel (CG). The DKME remained effective between 4 and over 12 months after being preserved in the cosmetic formula In Vitro. The DPKME@CG remained effective between 4 and over 12 months after being preserved in the cosmetic formula In Vitro. Significant improvements were also observed in skin glow (skin luster, skin hydration and elasticity, skin surface evenness, improved skin porosity, transepidermal water loss, and treatment of edema, erythematous boils, and vesicles). At week 12, most subjects and investigators reported an improvement in overall appearance with treatment, with a 100% improvement rate. No serious adverse reactions were observed.Conclusions: Our study provides real-world insights into the efficacy and safety of date kernel extract in improving facial skin quality in women, evaluated through objective and subjective assessments.
Zaghloul et al. (Fri,) studied this question.