Scanning Electron Microscopy Observations of Pseudomonas sp. v1

This protocol outlines a detailed method for preparingPseudomonas sp. for scanning electron microscopy (SEM). A combination of glutaraldehyde fixation, ethanol dehydration, dual-step cryopreservation (propane and liquid nitrogen), and freeze-drying ensures the preservation of bacterial ultrastructure. Gold sputter coating enhances conductivity, allowing high-resolution SEM imaging. This method is optimized to avoid morphological distortions and ensure structural clarity during imaging.