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This protocol describes theBIOLOG GEN III Assayused to analyze themetabolic and chemical sensitivity profilesof Pseudomonas sp. The assay assessescarbon source utilization (columns 1–9) and chemical sensitivity (columns 10–12)usingBIOLOG GEN III microplates. The procedure includesbacterial culture preparation, inoculum standardization, microplate inoculation, incubation, and data collectionusing aSynergy plate reader.
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Ali et al. (2025) studied this question.
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