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This is a Experimental Study study.

September 20, 2025Open Access

A Two-Phase Enzymatic Cascade for Indigo Synthesis From Anthranilate

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Enzymatic synthesis route achieved 183 mg/L indigo from anthranilate, suggesting bio-based alternatives for dyeing.

A titer of 183 mg/L indigo was achieved through a five-step enzymatic pathway derived from anthranilate.
The methodology faced challenges, including byproduct formation during ZmBX1 uptake of indole-3-glycerol phosphate.
Stepwise optimization and process engineering were crucial in enhancing the indigo titer for sustainable dye synthesis.
Despite promising results, issues with yield improvement remain, indicating further optimization is needed for economic feasibility.

Boer et al. (2025) studied this question.

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