This protocol details sample generation and fixation methods for spatial transcriptomics in engineered tissues, suggesting enhanced analysis capabilities.
Spatial transcriptomics is a powerful tool for investigating how cellular composition and spatial relationships influence cell behavior. Here, we present a protocol for spatial transcriptomic profiling of 2D engineered tissues and cell cultures that are not compatible with standard embedding and sectioning. We detail steps for sample generation, fixation, and staining to integrate with Visium HD spatial technology. This protocol provides a flexible solution for sample generation for spatial transcriptomic analysis.
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Helms et al. (2025) studied this question.
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