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This is a Quantitative Study study.

September 12, 2025Veterinary WorldOpen Access

Comparative molecular detection and phylogenetic analysis of Babesia canis vogeli in naturally infected dogs using two 18S rRNA primer sets in Khon Kaen, Thailand

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Authors

CKClara Ancilia Pramita KusumasriKhon Kaen University PPPatchara PhuektesKhon Kaen University NFNumfa FungbunKhon Kaen University

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Implication

Study demonstrates superior diagnostic sensitivity using two 18S rRNA primer sets for canine babesiosis, highlighting the significance of in-depth molecular analysis.

Key Points

PCR increased detection of babesia canis vogeli to 14.47%, showing 100% sensitivity with babf/babc primer set, compared to 11.9% from microscopy.
Both primer sets achieved 100% specificity and positive predictive value, with an equal limit of detection of 105 DNA copies.
Phylogenetic analysis revealed minimal intraspecific variation of babesia canis vogeli across global isolates, indicating closely related strains.
The babf/babc primer set proved superior for specific diagnosis, while bab7/bab9 was effective for broader screening of both babesia and hepatzoon canis.

Cite This Study

Kusumasri et al. (2025) studied this question.

synapsesocial.com/papers/68d46cc631b076d99fa68efb https://doi.org/10.14202/vetworld.2025.2663-2677

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Implication

Study demonstrates superior diagnostic sensitivity using two 18S rRNA primer sets for canine babesiosis, highlighting the significance of in-depth molecular analysis.

Comparative molecular detection and phylogenetic analysis of Babesia canis vogeli in naturally infected dogs using two 18S rRNA primer sets in Khon Kaen, Thailand

Discussion

Member takes

Implication

Key Points

Cite This Study

Comparative molecular detection and phylogenetic analysis of Babesia canis vogeli in naturally infected dogs using two 18S rRNA primer sets in Khon Kaen, Thailand

Discussion

Member takes

Implication

Key Points

Cite This Study

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