This study demonstrates that auxin significantly alters growth in Saccharomyces cerevisiae, suggesting important controls for auxin-inducible degradation.
Auxin-inducible degradation is an increasingly popular protein-targeting reverse genetics approach. Use of this method has revolutionized the types of questions cell and molecular biologists can answer, however, a growing number of studies point to auxin alone impacting different cellular phenotypes. This study investigated the impact of different medium and auxin combinations on Saccharomyces cerevisiae growth. We observed that both natural and synthetic auxin (Indole-3-acetic acid (IAA) and 1-Naphthaleneacetic acid (NAA) respectively) impaired yeast growth in nutrient minimal but not nutrient rich media. This finding was true across different yeast strains or with an intact auxin-inducible degradation system. Ultimately, this study highlights the need for proper controls when using auxin-inducible degradation.
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Nnabuenyi et al. (2025) studied this question.
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