Comparative spectral analysis of Ferrum phosphoricum 6X with water: Insights by ultraviolet visible spectrophotometry

Objectives: This study aimed to evaluate the stability, potency, and efficacy of Ferrum phosphoricum 6X using UV-visible spectrophotometry, by detecting the presence of particles and determining the maximum absorbance wavelength (λmax) in comparison with distilled water. Material and Methods: UV-visible spectrophotometric analysis was conducted for Ferrum phosphoricum 6X and distilled water across a wavelength range of 300–800 nm. Absorbance spectra were recorded to identify significant peaks and to confirm the presence of particulate matter. Results: The analysis revealed a distinct absorbance peak at 600 nm for Ferrum phosphoricum 6X, indicating the presence of particulate matter within the sample. In contrast, distilled water showed no such peak within the same range. Conclusion: The findings demonstrate that UV-visible spectrophotometry is a powerful and effective tool for validating the stability and potency of homoeopathic remedies such as Ferrum phosphoricum 6X. This approach supports quality control and assures therapeutic reliability in homoeopathic formulations.