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September 23, 2025International Journal of Molecular SciencesOpen Access

Transcriptomic Analysis of Osmotic Stress-Tolerant Somatic Embryos of Coffea arabica L. Mediated by the Coffee Antisense Trehalase Gene: A Marker-Free Approach

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Authors

EVEliana Valencia-LozanoUniversidad Nacional Autónoma de MéxicoABAarón BarrazaGenesis Biotechnologies (United States)JIJorge E. IbarraPontificia Universidad Católica de Chile

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Implication

Transcriptomic analysis demonstrates osmotic stress tolerance in coffee plants via trehalase gene silencing, suggesting enhanced drought resilience.

Key Points

  • Osmotic stress-tolerant somatic embryos displayed higher secondary SE capacity and increased plant robustness.
  • Somatic embryogenic lines showed significant trehalose accumulation and down-regulated trehalase, suggesting improved osmotic stress response.
  • A transcriptome analysis identified key biological pathways associated with osmotic stress tolerance in coffee somatic embryos.
  • Higher photosynthetic rates and improved water-use efficiency were noted, highlighting drought adaptation in the modified lines.

Cite This Study

Valencia-Lozano et al. (2025) studied this question.

synapsesocial.com/papers/68d473b531b076d99fa6c6dahttps://doi.org/10.3390/ijms26189224
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