Vitamin B-6 Reduces Nitrite Oxide Levels, Interleukin-6, and Plasminogen Activator Inhibitor-1 in the Small Intestines and Reduces the Incidence of Post-Laparotomy Intraperitoneal Adhesions in Wistar Rats: A Randomized Trial

Objective: Intraperitoneal adhesions occur in approximately 95% of cases after laparotomy. Vitamin B6 has a few effects, including the inhibition of nitric oxide (NO), suppression of Interleukin-6 (IL-6) production, and decreased fibrinolytic capacity. This study was conducted to determine whether intramuscular administration of Vitamin B6 reduces levels of NO, IL-6, and plasminogen activator inhibitor-1 (PAI-1) and the incidence of intraperitoneal adhesions.Material and Methods: This experimental study was conducted with a Randomized Post-Test Only Control Group Design. This study used 20 male Wistar rats divided into 2 groups (treatment and control groups). The treatment group received an intramuscular injection of 10 mg/kg/day of Vitamin B6. NO, IL-6, and PAI-1 were measured using the Enzyme-linked Immunosorbent Assay (ELISA) method. Intraperitoneal adhesions were determined based on the Zuhkle criteria.Results: The incidence of adhesion was 30% in the treatment group and 100% in the control group. NO, IL-6, PAI-1 levels, and the incidence of adhesion in the treatment group were significantly lower in the treatment group (p-value 0.002; <0.001; <0.001; 0.002, respectively). Furthermore, NO, IL-6, and PAI-1 levels were significantly lower in subjects without adhesions compared with those with adhesions (p-value<0.001, respectively). Vitamin B6 also decreased the risk of adhesions 3.3 times compared to the control group (HR 3.3; 95% CI: 1.29-8.59).Conclusion: There was a significant reduction in NO, IL-6, and PAI-1 levels after intramuscular administration of Vitamin B6, which contributed to a lower incidence of intraperitoneal adhesions.