Supranutritional Selenium Supplementation Modulated Lipopolysaccharide Provoked Inflammatory Injury in Colon of Goat Fed Grain Rich Diet

The current research was designed to examine the role of extra selenium (Se) supplemented against high grain diet induced stress mediated inflammatory response leading to epithelial injury in the colon of goat. Eighteen goats were divided into three dietary groups: one received a low-grain diet (LG; grain to forage ratio of 35:65), another was fed a high-grain diet (HG; grain to forage ratio of 65:35), and the third group was provided a high-grain diet supplemented with selenium (HG-Se; 65:35 plus 0.5 mg Se per kg of diet). At the end of tenth week experiment period, compared with low grain, high grain diet elevated (P < 0.05) colonic and plasma lipopolysaccharide (LPS) concentration. Colonic epithelial injury accompanied with enhanced (P < 0.05) melondialdehyde (MDA) and diminishing (P < 0.05) the activities of total antioxidant status (T-AOS) was observed in high grain diet than low grain diet. Moreover, the HG diet led to a significant increase (P < 0.05) in the levels of plasma acute phase proteins (APPs), including serum amyloid A (SAA), lipopolysaccharide-binding protein (LBP) and haptoglobin (Hp), accompanied by changes in the colonic mRNA expression of immune-related genes such as toll-like receptor 4 (TLR-4), cluster of differentiation 14 (CD-14), tumor necrosis factor-alpha (TNF-α), nuclear factor kappa B (NF-κB), and interleukins IL-1, IL-6, IL-10, and IL-13. Conversely, surplus Se supplementation lessens LPS level in colon and blood thus alleviated colonic epithelial injury in high grain-Se diet. Besides, Se supplemented high grain diet modulated plasma APPs and inflammatory genes in goat colon. In conclusion, high grain diet-induced colonic inflammation was associated with TLR-4- MYD88 signaling pathway. Conversely, extra Se supplementation alleviated high grain diet induced stress mediated inflammatory response through TLR-4- MYD88 signaling pathway thus reduced colonic injury in the goats.